In this study, a gel cleaning experiment was conducted to stably remove the fixative (PVAc) used in the past conservation treatment of the mural painting of the Payathonzu temple. This study prepared mock-up samples reflecting the material properties and conservation status of the mural painting of the Payathonzu temple, and conducted an experiment about the applicability of 6 types of gel cleaners to discern potential changes when they are applied to the actual mural painting and to review their stability. This study found that the gel cleaner mixed with dimethyl carbonate had a superior removal effect to the gel cleaner mixed with acetone. Furthermore, for the same solvent, the gelling agent showed the removal effect in the order of Nevek® > Carbopol®980 > Laponite®RD. Upon examining change patterns after applying the cleaners, this study found that the cleaners with a superior removal power had a similar trend to that in removal effect evaluation in a previous study. In actually applying to the mural painting, however, the cleaners showed some inconsistent results with those in removal power evaluation, and it was confirmed that these results were attributable to the properties of the cleaners interacting with the properties of the mural painting and the extent of penetration of the fixative. It was also found that not only the conditions of the cleaners but also the material and painting technique characteristics of the mural could have a huge effect during the removal of the fixative. What is most important in removing the fixative applied to the mural painting of the Payathonzu temple is to ensure the stability of its painting layer by using a cleaning method that is controllable by the person treating the painting, and then, the properties that can help to effectively remove the fixative are required. At a time whenthere are few related studies in Korea, this study and its method may serve as a reference for future research on the removal of the fixative applied to a mural.